pearson correlation analysis Search Results


pearson’s correlation analysis  (GraphPad Software Inc)
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GraphPad Software Inc pearson’s correlation analysis
(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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pearson's correlation and linear regression analysis  (GraphPad Software Inc)
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(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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correlogram analysis  (Federation of European Neuroscience Societies)
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Federation of European Neuroscience Societies correlogram analysis
(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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pearson correlation analysis in graphpad 8  (GraphPad Software Inc)
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(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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pearson correlation analysis  (RStudio)
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RStudio pearson correlation analysis
(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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pearson correlation analysis  (SAS institute)
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SAS institute pearson correlation analysis
(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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pearson correlation analysis  (DuPont de Nemours)
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DuPont de Nemours pearson correlation analysis
(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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pearson correlation analyses  (OriginLab corp)
90
OriginLab corp pearson correlation analyses
(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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pearson's r in graphpad prism 6.0  (GraphPad Software Inc)
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(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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means comparison and pearson correlation analysis  (SAS institute)
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(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) <t>Pearson’s</t> correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.
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(A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) Pearson’s correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.

Journal: bioRxiv

Article Title: Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production

doi: 10.1101/2023.11.07.566074

Figure Lengend Snippet: (A) Histological analysis of MTHFD2 protein expression in fibrotic and nonfibrotic areas of paraffin-embedded lung sections from a patient with idiopathic pulmonary fibrosis. (B) Western blot analysis of MTHFD2 protein expression in mouse lung tissue either prior to, or 14 or 21 days after bleomycin instillation. (C) Pearson’s correlation of MTHFD2 mRNA level and forced vital capacity before bronchodilator (FVC-pre-BD) as percentage of what was predicted for each patient. Data is from GSE32537. (D) Western blot analysis of collagen and α-smooth muscle actin protein expression in normal human lung fibroblasts (NHLFs) treated with TGF-β for the indicated intervals in the presence or absence of DS18561882 (5μM). (E-G) Analysis of cellular (E) glycine, (F) serine, and (G) proline in NHLFs after labeling with 2,3,3-D3-Serine in the presence or absence of TGF-β and DS18561882. (H) Relative levels of media formate content in NHLFs treated with TGF-β or left untreated in the presence or absence of DS18561882. (I) Representative coronal microCT images of mice prior to bleomycin instillation (Day 0), 7 days after bleomycin instillation (Day 7), and 14 days after bleomycin instillation (Day 14). Mice began receiving DS18561882 (125 mg/kg) or vehicle 8 days after bleomycin instillation. (J) Quantification of aerated lung volumes from microCT-scanned mice treated as in (I) (mean ± SEM). (K) Histological analysis of mouse lung tissue on day 21 after bleomycin instillation. Beginning on day 8, mice received daily IP injections of either DS18561882 or vehicle. (L) Hydroxyproline content of mouse lungs on day 21 after bleomycin or vehicle (saline) instillation. Beginning on day 8, mice received either DS18561882 or vehicle IP, daily (mean ± SEM). * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Available clinical data was correlated to individual gene expression profiles using Pearson’s correlation analysis using GraphPad Prism v10.0.2.

Techniques: Expressing, Western Blot, Labeling, Saline